Sisebenzisa iikuki ukuphucula amava akho.Ngokuqhubeka nokukhangela le ndawo, uyavumelana nokusebenzisa kwethu iikuki.Iinkcukacha ezongezelelweyo.
Abantu kwihlabathi liphela basebenzisa iziselo zamandla ukuphucula ukugxila kwabo kunye nemveliso.Enye yezona ndlela zisebenzayo zokuhlalutya ezi ziselo yi-capillary electrophoresis.Eli nqaku livavanya amandla kunye nokufaneleka xa kuthelekiswa nezinye iindlela ezifana ne-chromatography yolwelo.
Uninzi lweziselo zamandla zenziwe kwiikhompawundi ezityebileyo zecaffeine, kubandakanya icaffeine kunye neglutamate.I-caffeine yi-alkaloid evuselelayo efumaneka kwiintlobo zezityalo ezingaphezu kwama-63 kwihlabathi jikelele.I-caffeine ecocekileyo iyakrakra, ayinancasa, iqinile emhlophe.Ubunzima bemolekyuli yecaffeine 194.19 g, indawo yokunyibilika 2360°C.I-caffeine i-hydrophilic kwiqondo lokushisa kwegumbi kunye noxinzelelo olukhulu lwe-21.7 g / l ngenxa ye-reactivity yayo ephakathi.
Iziselo ezithambileyo ziinkqubo ezintsonkothileyo eziqulethe izithako ezininzi ezahlukeneyo, zombini kunye ne-organic.Ukwahlula iitshekhi kubalulekile ukubona ngokuchanekileyo kunye nokuvavanya ezinye iindidi ezahlukeneyo caffeine kunye benzoates.Eyona ndlela ixhaphakileyo esetyenziselwa ukuvavanya ukwahlula okudityanisiweyo yi-liquid chromatography (LC).
I-chromatography yolwelo kuxelwe ukuba isetyenziselwa ukwahlula phakathi koluhlu olubanzi lweeamolekyuli zezinto eziphilayo, ukusuka kungcoliseko oluncinci lwemolekyuli ukuya kwi-peptides ye-antimicrobial.Ujongano olwahlukileyo phakathi kwezigaba ezihambayo nezimileyo zeemolekyuli kwisampulu ziphantsi kokwahlulwa kwechromatography yolwelo.Ukuqina kwebhondi, ngcono i-molecule ibambe indawo yayo.
Enye indlela kwiinkqubo ze-HPLC kukwahlulwa nge-silica capillary electrophoresis emxinwa, esebenzisa indawo yombane ukwahlula iikhompawundi kumaqela ahlukeneyo ekhemikhali kwisampulu enye.I-CE inokwahlulwa kwiindlela ezininzi zokwahlula ngokuxhomekeke kwii-capillaries kunye ne-ion ezisetyenzisiweyo.
Indlela ye-capillary electrophoresis iluncedo kakhulu ekuvavanyeni ukutya kunye nesiselo ngenxa yeenzuzo zayo zesampulu ephantsi kunye nokusetyenziswa kwe-reagent, ixesha elifutshane lokuhlalutya, iindleko eziphantsi zokusebenza, isisombululo esiphezulu, ukusebenza kakuhle kokususa, ukulula kovavanyo kunye nophuhliso lwenkqubo ekhawulezayo.
Indlela yokwahlula i-electrophoresis isekelwe kwiintshukumo ezahlukeneyo zeeyoni zemichiza kwiseli ye-electrolytic phantsi kwesenzo sentsimi yombane esetyenzisiweyo.Xa kuthelekiswa nezixhobo ezintsonkothileyo zechromatography yolwelo, izixhobo ze-capillary electrophoresis zilula.Umbhobho odibanisayo onobubanzi bangaphakathi obuyi-25-100 m kunye nobubanzi obuyi-20-100 cm udibanisa iiseli ze-buffer ezimbini, apho amandla ombane aphezulu (0-30 kV) anikezelwa ngee-conductors kwaye i-electrolysis circuit esebenzayo ilayishwa njenge umthwali ohlawulisiweyo.
Ngokuqhelekileyo, i-anode ithathwa njenge-capillary inlet kwaye i-cathode ithathwa njenge-capillary outlet.Isixa esincinci sesampulu sitofwa nge-hydraulically okanye ngombane kwicala le-anode ye-capillary.I-motorized infusion yenziwa ngokutshintshela i-buffer reservoir kunye ne-vial yesampuli kunye nokusebenzisa umbane wangoku ixesha elithile ukuvumela amasuntswana ukuba ahambe kwi-capillary.
I-Hydrostatic infusion ihambisa isampuli ngokusekwe kuxinzelelo lokuhla phakathi kwe-inlet kunye ne-outlet ye-capillary, kwaye isixa sesampulu esitofelweyo simiselwa kukuhla koxinzelelo kunye nobukhulu be-polymer matrix.Emva kokuba isampuli ilayishiwe, inxalenye yesampuli iqokelela kwi-capillary opening.
Iimpawu zokuhlukana kweendlela ze-capillary electrophoresis zinokulinganiswa ngeendlela ezimbini: isisombululo sokuhlukana, ii-Rs, kunye nokusebenza kakuhle kokuhlukana.Isisombululo sabahlalutyi ababini sibonisa indlela abanokuthi baphumelele ngayo ukwahlula omnye komnye.Xa lilikhulu ixabiso le-Rs, kokukhona ivakala ngakumbi incopho ethile.Isisombululo sokwahlula silinganisa ukusebenza kakuhle kokwahlula kwaye sivavanya ukuba ngaba uhlengahlengiso kwindawo yovavanyo lunokubangela ukwahlulwa kwemixube.
Ukusebenza kakuhle kokwahlula i-N yindawo ecingelwayo apho izigaba ezibini zilingana kunye, ezimelwe ngamanani eepaneli ezahlukeneyo, kuxhomekeke kumgangatho wekholomu kunye nolwelo.
Uphononongo olutsha olupapashwe kwiNgqungquthela yeHlabathi yezoLimo kunye noZinzo lujolise ekuphandeni amandla e-capillary electrophoresis ukuchonga i-nitrogenous compounds kunye ne-ascorbic acid kwiziselo, kunye nefuthe lezinto eziguquguqukayo ze-electrophoresis kwiipropati zobungakanani bendlela.
Izinto ezilungileyo ze-capillary electrophoresis ngaphezu kwe-chromatography yolwelo olusebenzayo oluphezulu lubandakanya iindleko eziphantsi zophando kunye nokuhambelana kokusingqongileyo, kunye novavanyo lwe-asymmetric organic acid okanye iincopho zesiseko.I-Capillary electrophoresis inikezela ukuchaneka okwaneleyo kokuchongwa kweekhemikhali ze-labile kwiimatriki eziyinkimbinkimbi kunye neeparitha ezithile ezisisiseko (ukusasazwa kwentlama kwi-buffer ehambayo, ukuqinisekisa ukuba i-homogeneity yokwakheka kwe-buffer, ukuqina kobushushu bemigangatho eyahlulayo).
Isishwankathelo, nangona i-capillary electrophoresis ineengenelo ezininzi ngaphezulu kokusebenza okuphezulu kolwelo lwechromatography, ikwanazo nezinto ezingeloncedo njengamaxesha amade ohlalutyo.Uphando olongezelelweyo kufuneka lwenziwe ukufumana iindlela zokuphucula le ndlela.
URashid, SA, Abdulla, SM, Najeeb, BH, Hamarashid, SH, & Abdulla, OA (2021). URashid, SA, Abdulla, SM, Najeeb, BH, Hamarashid, SH, & Abdulla, OA (2021).URashid, SA, Abdullah, SM, Najib, BH, Hamarasheed, SH, kunye no-Abdullah, OA (2021).URashid SA, Abdullah SM, Najib BH, Hamarasheed SH kunye no-Abdulla OA (2021).Ukumiselwa kwe-caffeine kunye ne-sodium benzoate kwiziselo zamandla ezivela kumazwe angaphandle kunye nezasekhaya zisebenzisa i-HPLC kunye ne-spectrophotometer.Uluhlu lweNkomfa ye-IOP: iSayensi yoMhlaba kunye nokusiNgqongileyo.Ifumaneka apha: https://iopscience.iop.org/article/10.1088/1755-1315/910/1/012129/meta.
I-ALVES, AC, MEINHART, AD, & FILHO, JT (2019). I-ALVES, AC, MEINHART, AD, & FILHO, JT (2019).I-ALVES, AS, MEINHART, AD, kunye ne-FILHO, JT (2019).I-ALVES, AS, MEINHART, AD kunye ne-FILHO, JT (2019).Ukuphuhliswa kwendlela yokuhlalutya ngaxeshanye ye-caffeine kunye ne-taurine kumandla.Inzululwazi yoKutya kunye neTeknoloji.Ifumaneka apha: https://www.scielo.br/j/cta/a/7n534rVddj3rXJ89gzJLXvh/?lang=en
UTuma, uPiotr, uFrantisek Opekar, kunye noPavel Dlouhy.(2021).I-electrophoresis ye-Capillary kunye ne-microarray ene-non-contact conductivity determination for food and drink analysis.chemistry yokutya.131858. Ifumaneka apha: https://linkinghub.elsevier.com/retrieve/pii/S0308814621028648.
Khasanov, VV, Slizhov, YG, & Khasanov, VV (2013). Khasanov, VV, Slizhov, YG, & Khasanov, VV (2013).Khasanov VV, Slizhov Yu.G., Khasanov VV (2013).Khasanov VV, Slizhov Yu.G., Khasanov VV (2013).Uhlalutyo lweziselo zamandla nge-capillary electrophoresis.Ijenali ye-Analytical Chemistry.Ifumaneka apha: https://link.springer.com/article/10.1134/S1061934813040047.
Umlandeli, u-KK (207).Uhlalutyo lwe-Capillary lwe-preservatives kwiziselo zamandla.IYunivesithi yaseCalifornia Polytechnic State, ePomona.Ifumaneka apha: https://scholarworks.calstate.edu/concern/theses/mc87ps371.
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U-Ibtisam uphumelele kwi-Islamabad Institute of Space Technology ngesidanga se-bachelor kubunjineli be-aerospace.Ngethuba lomsebenzi wakhe wokufunda, uye wabandakanyeka kwiiprojekthi ezininzi zophando kwaye uye waququzelela ngempumelelo imisebenzi emininzi engaphandle kwekharityhulam efana ne-International World Space Week kunye neNkomfa yeZizwe ngeZizwe yobuNjineli be-Aerospace.U-Ibtisam uphumelele ukhuphiswano lwesincoko solwimi lwesiNgesi ngeentsuku zakhe zomfundi kwaye ebesoloko ebonisa umdla wophando, ukubhala kunye nokuhlela.Kungekudala emva kokuphumelela, wajoyina i-AzoNetwork njenge-freelancer ukuphucula izakhono zakhe.U-Ibtisam uthanda ukuhamba, ngakumbi emaphandleni.Ebesoloko engumntu othanda imidlalo kwaye ebekonwabela ukubukela intenetya, ibhola ekhatywayo kunye neqakamba.Uzalelwe ePakistan, u-Ibtisam unethemba lokukhenketha ilizwe ngenye imini.
Abbasi, Ibtisam.(Ngomhla we-4 ka-Epreli 2022).Uhlalutyo lweziselo zamandla nge-capillary electrophoresis.AZOM.Ibuyiselwe nge-13 ka-Okthobha, 2022 ukusuka ku-https://www.azom.com/article.aspx?ArticleID=21527.
Abbasi, Ibtisam."Uhlalutyo lweZiselo zaMandla ngeCapillary Electrophoresis".AZOM.Ngo-Oktobha 13, 2022.Ngo-Oktobha 13, 2022.
Abbasi, Ibtisam."Uhlalutyo lweZiselo zaMandla ngeCapillary Electrophoresis".AZOM.https://www.azom.com/article.aspx?ArticleID=21527.(Ukusukela nge-13 ka-Okthobha ngo-2022).
Abbasi, Ibtisam.2022. Uhlalutyo lweziphuzo zamandla nge-capillary electrophoresis.I-AZoM, ifunyenwe nge-13 ka-Okthobha 2022, https://www.azom.com/article.aspx?ArticleID=21527.
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